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Table 1 Rate constants defined in Figure 1 for thioester exchange and hydrolysis at different temperatures and pHs.

From: Dynamic combinatorial chemistry at the phospholipid bilayer interface

starting material

pH

T [°C]

k 1 [M-1s-1]

k -1 [M-1s-1]

k h1 [s-1]

k 1 /k -1

K [a]

1a

7.0

40

0.868 × 10-2 ± 1.8 × 10-4

3.51 × 10-2 ± 1.3 × 10-3

5 × 10-5 ± 2 × 10-5

0.25

0.29

 

8.0

40

7.58 × 10-2 ± 6.8 × 10-3

18.5 × 10-2 ± 2.3 × 10-2

1.1 × 10-4 ± 1 × 10-5

0.41

0.43

  

30

2.93 × 10-2 ± 3 × 10-4

6.42 × 10-2 ± 1.1 × 10-3

6 × 10-5 ± 1 × 10-5

0.46

0.46

  

20

1.22 × 10-2 ± 1 × 10-4

2.92 × 10-2 ± 4 × 10-4

2 × 10-5 1 × 10-5

0.42

0.44

1b

7.0

40

1.08 × 10-2 ± 2 × 10-4

1.00 × 10-2 ± 8 × 10-4

1.64 × 10-3 ± 3 × 10-5

1.08

1.10

 

8.0

40

8.13 × 10-2 ± 1.4 × 10-3

6.12 × 10-2 ± 2.1 × 10-3

2.29 × 10-3 ± 3 × 10-5

1.33

1.28

  

30

4.09 × 10-2 ± 1.2 × 10-3

4.01 × 10-2 ± 2.1 × 10-3

8.0 × 10-4 ± 4 × 10-5

1.02

1.08

  

20

1.54 × 10-2 ± 2 × 10-4

1.69 × 10-2 ± 6 × 10-4

3.2 × 10-4 ± 2 × 10-5

0.91

0.92

  1. a Determined from the concentrations of thiol and thioesters observed in the equilibrated libraries.