Performance of the amplification module. (A) Scheme for the fluorescence reporter. Color coding is as in Figure 1. (B and C) Kinetics of fluorescent signal accumulation, highlighting the effects of Trigger addition (B) and Fuel addition (C). (D) Fluorescent signals for all samples at the 1 hr time point. Color coding is the same as in (B) and (C). The residual fluorescent signal due to incomplete qunching of 100 nM fReporter was arbitrarily defined as 1 RFU. The error bars represent the standard deviation of the fluorescent signals from two identical reactions that were carried out and measured in two wells of a 384-well plate. Whenever present, the final concentrations of fReporter, Gate and Fuel in these experiments were 100 nM, 100 nM, and 300 nM, respectively. The concentrations of Trigger in different reactions were as indicated.