Figure 5
Polycondensation in mixtures. Total nucleotide concentration is 20 mM. 20% polyacrylamide denaturing gel is stained with SybrGold® gel stain for 20 min. Line M is a marker line. (A) nCGP polymerization in presence of nCAp. Ratio of the two dinucleotides varies as shown in the picture. Reaction is analyzed after 48 h of reaction. (B) nCGp polymerization in presence of nTGp. Ratio of the two dinucleotides varies as shown in the picture. Reaction is analyzed after 48 h of reaction. (C) nCGp + nCAp + nTG + nTAp at 1:1:1:1 ratio gives strands approximately 50 bases long. All four dinucleotides are incorporated.