Enzymatic synthesis of DNA employing pyrophosphate-linked dinucleotide substrates

Abstract

Background

One of the remaining questions in the understanding of the origin of Nature's information system is the way the first nucleic acids have been synthesized. This could have been realized using nucleoside triphosphates or imidazolides of nucleoside monophosphates as building blocks. Alternatively, dinucleoside pyrophosphates could have been used for this purpose. The advantage of using building blocks, composed of pyrophosphate-linked dinucleotides, could be that exponential growth of initial information (dinucleotides) without product inhibition might become possible.

Results

Herein, we demonstrate that dinucleoside pyrophosphates are able to act as substrate for HIV-1 RT and several thermostable DNA polymerases. In single incorporation assay, compound dAppdA was able to give a 100% conversion to the (P+1) strand by Therminator DNA polymerase and at a substrate concentration above 100 μM. Full-length elongation was obtained in a chain elongation experiment, with over 95% yield of (P+7) product by Taq and Vent (exo^-) DNA polymerase. Interestingly, using heterodimer dAppdT addition of either nucleotide component of the dinucleotide substrate into the DNA chain can occur, which is defined by the template program.

Conclusions

This study shows that dinucleoside pyrophosphates can be considered as a new type of substrate for polymerases in the template-directed DNA synthesis. Using heterodimers as substrate, theoretically, it is possible to synthesize DNA enzymatically using two building blocks (dAppdT and dGppdC) instead of four. Given the poor Km value for the nucleotide incorporation, evolution of polymerases will become necessary to make this process of practical use.

Background

The phosphorylation of nucleosides and nucleoside monophosphates under prebiotic conditions and leading to the formation of nucleoside diphosphates and nucleoside triphosphates was studied in the seventies of the previous century [1–5]. These studies were performed based on the observation that four nucleoside triphosphates are used as building blocks for the synthesis of each natural information system (DNA and RNA). In the context of the question "why this and not that", it is not unreasonable to ask the question why Nature uses four reagents to synthesize information systems and not two, composed of, for example, dinucleoside pyrophosphates. Dinucleoside pyrophosphates are not alien to cellular biology, demonstrated by the coenzyme function of NAD⁺ (Figure 1) and NADP⁺ in redox reactions. Moreover, NAD⁺ is used by poly(ADP-ribose) polymerase to synthesize PAR (Poly ADP ribose) [6] polymers using nicotinamide as leaving group. The linking group between the ribosylated adenine and nicotinamide units is pyrophosphate. Another example is that of the ligases. Phosphodiester bond formation between a 3'-hydroxyl group and a 5'-phosphoryl group in nicked DNA by ligase is running via the formation of a nucleotide pyrophosphate intermediate [7].

Figure 1

Structure of NAD⁺.

Dinucleotides are chemically easily available, demonstrated by the observation that the reaction of ImpA with glycolic acid in the presence of divalent metal ions may lead to AppA formation [8]. We also observed that L-Asp-dTMP may dimerize in solution to give dTppdT (unpublished results). The use of pyrophosphate linked nucleotides as building blocks for nucleic acid synthesis has not been investigated so far.

Indeed, dNTPs, consisting of dATP, dCTP, dGTP and dTTP, are standard natural substrates of all DNA polymerases. The four dNTPs are used according to the base pairing rules to extend the primer. In the process of this polymerization, the enzyme catalyzes the phosphoester bond formation between the 3'-hydroxyl group of the primer and dNTP α-phosphate, and makes a step along the 5' → 3' direction while releasing a pyrophosphate molecule (PPi) as leaving group. In recent years, our group proved that some selected structures like amino acid phosphoramidates are able to be processed as substrates by polymerase in the enzymatic-catalyzed DNA synthesis [9–16], despite the fact that a P-N bond is much more stable than a P-O bond. For example, in a primer-template assay iminodipropionic acid-dAMP phosphoramidate (IDP-dAMP) is able to give a 91% conversion to the (P+1) strand at 50 μM by HIV-1 RT [16]. In this case, the pyrophosphate leaving group (PPi) is replaced by an iminodipropionic acid group, which shows similar binding behaviours to the primer-template duplex and enzyme as pyrophosphate. This work is now extended by the demonstration that dinucleoside pyrophosphates (Table 1) are able to act as a new type of substrate by polymerase in the template-directed DNA synthesis, and this observation is relevant to the question about the origin of life of the present natural information system. The substrate properties towards various polymerases, as well as the stability study are discussed.

Table 1 Overview of the dinucleoside pyrophosphates used in this study.

Results and Discussion

Synthesis of dinucleoside diphosphates

The synthesis of dinucleoside pyrophosphates I-VII was carried out using the corresponding nucleoside-5'-phosphorimidazolide as intermediates. Deoxynucleoside monophosphate was first converted to its phosphorimidazolide according to method described by Orgel and colleagues [17], then the phosphorimidazolide is reacted with the corresponding nucleoside monophosphate in 0.2 M N-ethylmorpholine buffer (pH = 8~8.5), using Pd(NO₃)₂ or ZnCl₂ as catalyst (Scheme 1). After completion, appropriate amount of EDTA (0.25 M) solution was added to disassociate the nucleotide-metal complex, followed by purification with HPLC (reverse phase C18 column) to provide the pure dinucleoside pyrophosphates.

Scheme 1

Synthesis of compound I-VII. Reagents and conditions. (a) Ph₃P, imidazole, dipyridyl disulfide, DMSO, rt.; (b) dNMP (N = A, T, G, C), 0.25 M N-ethylmorpholine buffer (pH = 8~8.5), Pd(NO₃)₂/ZnCl₂, rt.

Single nucleotide incorporation by HIV-1 RT

The substrate properties of compound I-VII catalyzed by HIV-1 RT, Taq, Vent (exo^-) and Therminator DNA polymerase were studied using the primer-template complexes as shown in Table 2.

Table 2 Overview of primer and template sequences used in the DNA polymerase reaction^[a]

Since HIV-1 RT is an error-prone polymerase with high mutation rate, it exhibits flexibility and high tolerance towards chemically modified nucleotide substrate [18, 19]. Therefore, we firstly select HIV-1 RT to perform the single nucleotide incorporation experiments in a primer-template assay. The building blocks were incubated at 37°C, with the appropriate primer-template complex and 0.025 U/μL of enzyme, samples were taken after 10, 20, 30, 60, and 120 min, and analyzed by polyacrylamide gel electrophoresis.

As shown in Figure 2a and 2b, compound I and II can be recognized smoothly by HIV-1 RT, and incorporated into the growing DNA stand resulting 100% and 96% conversion to the (P+1) strand at a concentration of 500 μM over a period of 2 h, respectively. Interestingly, using compound III as reagent, containing a deoxyadenylate and a thymidylate residue, addition of either nucleotide component of the dinucleotide substrate can occur by HIV-1 RT, which is defined by the template program. As shown in Figure 2c and 2d, compound III is able to insert a deoxyadenylate residue into the P₁T₁ with 48% conversion to the (P+1) strand (Figure 2c), and also compound III is able to insert a deoxythymidylate residue into P₁T₂ duplex with 32% conversion to the (P+1) strand (Figure 2d). Although the incorporation of compound III only gave us a moderate yield, it is interesting to observe that two different nucleotide residues (deoxyadenylate and deoxythymidylate) are able to be incorporated into the growing strand by a single molecule dinucleoside pyrophosphates instead of two classic dNTP building blocks. Based on the these observations, it is not unreasonable to hypothesize that it should be possible to replace the standard four dNTP building blocks with two dinucleoside pyrophosphate analogues in the enzymatic DNA polymerization reaction (e.g. PCR).

Figure 2

Single incorporation of compound I, II, and III by HIV-1 RT. [HIV-1 RT] = 0.025 U/μL, time points: 10, 20, 30, 60, and 120 min. a) Incorporation of compound I, [P₁T₁] = 125 nM; b) Incorporation of compound II, [P₁T₂] = 125 nM; c) Incorporation of compound III, [P₁T₁] = 125 nM; d) Incorporation of compound III, [P₁T₂] = 125 nM.

Compound IV with two deoxyguanylate residues demonstrates weaker substrate property to HIV-1 RT than the deoxyadenosine or deoxythymidine congeners, 43% conversion to the (P+1) strand was observed (Figure 3a). It is worthy mentioning that compound V and VI can be accepted as substrate by HIV-1 RT extending the primer with deoxyguanylate or deoxyadenylate residue, although not very efficient (28% conversion for compound V, 15% conversion for compound VI) (Figure 3b, c). Disappointingly, when trying to extend the primer with deoxycytidylate residue (compound V, VI VII) under the same conditions, no incorporation was detected (data not shown).

Figure 3

Single incorporation of compound IV, V and VI by HIV-1 RT. [HIV-1 RT] = 0.025 U/μL, time points: 10, 20, 30, 60, and 120 min. a) Incorporation of compound IV, [P₁T₄] = 125 nM; b) Incorporation of compound V, [P₁T₄] = 125 nM; c) Incorporation of compound VI, [P₁T₁] = 125 nM.

Single nucleotide incorporation by other DNA polymerases

After obtaining the single nucleotide incorporation results of compound I-VII with HIV-1 RT, we tested the single incorporation properties of compound I-VII with a selection of thermostable DNA polymerases.

Compound I demonstrates very good substrate properties towards Taq, Vent (exo^-), or Therminator DNA polymerase. As shown in Figure 4, compound I can obtain 87% conversion with Taq DNA polymerase (Figure 4a) and 100% primer extension with Vent (exo^-) DNA polymerase at 500 μM substrate concentration (Figure 4b). Remarkably, a maximal primer extension was observed by using Therminator DNA polymerase with substrate concentration above 100 μM (Figure 4c).

Figure 4

Single incorporation of compound I and II by Taq, Vent (exo^-) and Therminator DNA polymerase. Time points: 10, 20, 30, 60, and 120 min. a) Incorporation of compound I by Taq DNA polymerase, [P₁T₁] = 125 nM; b) Incorporation of compound I by Vent (exo^-), [P₁T₁] = 125 nM; c) Incorporation of compound I by Therminator, [P₁T₁] = 125 nM; d) Incorporation of compound II by Therminator, [P₁T₂] = 125 nM.

Compound II, which shows similar substrate property as compound I using HIV-1 RT as polymerase, can achieve 70% conversion to (P+1) strand at 500 μM substrate concentration in 60 min and 100% conversion at 1 mM by Therminator DNA polymerase (Figure 4d). However, compound II was not a substrate for Taq and Vent (exo^-) DNA polymerase, as no (P+1) product formation was observed at the concentrations ranging from 100 μM to 1 mM (data not shown).

We also evaluated the substrate properties of compound III-VII for Taq, Vent (exo^-) and Therminator DNA polymerase. Unfortunately, Taq and Vent (exo^-) DNA polymerase failed to incorporate compound III-VII into a growing primer strand (Table 3). After 2 h of polymerase reaction, no (P+1) product was observed at 1 mM substrate concentration (Table 3).

Table 3 Single incorporation results of compound III-VII by Taq, Vent (exo^-) and Therminator DNA polymerase.

Therminator is a mutant variant of the 9°N exo^- polymerase (Thermococcus species 9°N-7), in which the Ala 485 residue has been replaced with Leu residue. This enzyme demonstrated effective ability of recognition and incorporation of a number of modified nucleotides bearing unnatural nucleobase and sugar moieties [20]. Using compound III-VI as substrates, Therminator polymerase was able to insert deoxyadenylate, deoxythymidylate and deoxyguanylate residue into the DNA primer-template complex (Table 3) depending on the template program, however, it fails to incorporated the deoxycytidylate residue into the growing primer strand as observed for HIV-1 RT. The observed diversity in incorporation selectivity for various polymerases (HIV-1 RT, Taq, Vent (exo^-)) indicates the differences in tolerance of the active site of polymerase to the triphosphate modifications.

Primer extension experiments

So far, compound I show the best results in single nucleotide incorporation experiments towards various polymerases. We decided to further investigate the chain elongation property of compound I by HIV-1 RT, Taq, Vent (exo^-) and Therminator DNA polymerase, respectively. In this experiment a template overhang with seven thymidines and flanked by four non-thymidine nucleotides at the 3'-end was used. The building block was incubated at the appropriate temperature in a concentration of 500 μM, with primer P₁ and template T₃ complex and appropriate concentration of enzyme, samples were taken after 15, 30, 60, 90, and 120 min, and analyzed by polyacrylamide gel electrophoresis.

The chain elongation results of compound I with Vent (exo^-) polymerase is shown in Figure 5. The primer extension by HIV-1 RT is not very efficient, only three building blocks were extended (data not shown). In contrast, Taq and Vent (exo^-) DNA polymerase are able to achieve a full-length elongation, with over 95% yield of (P+7) product. Therminator polymerase can also reach the full-length elongation, however, not so sufficient as when using the two other thermostable polymerases (data not shown).

Figure 5

Elongation of P ₁ T ₃ (125 nM) by Vent (exo^-) polymerase (0.10 U/μl) and compound I. [dAppdA] = 500 μM, time points: 15, 30, 60, 90, and 120 min.

Furthermore, we investigated the chain elongation property of the compound containing two different residues. As the deoxycytidylate residue was not a substrate for all the four tested enzymes, compound III was explored as substrate in this experiment and P₁T₆ was used as primer-template duplex (with a mixed AT sequence in the overhanging part of template) and HIV-1 RT as enzyme. Compound III was able to be polymerised by HIV-1 RT, however, only (primer + 3) product can be obtained (Figure 6). When the kinetics for incorporation of dTMP and dAMP is different, it is difficult to select appropriate reaction conditions for the chain elongation reaction using dAppdT as reagent and poly (AT)_n as template, avoiding misincorporation.

Figure 6

Elongation into P ₁ T ₆ (125 nM) by compound III. Time points: 15, 30, 60, 90, and 120 min; [HIV-1 RT] = 0.025 U/μL.

Incorporation kinetics

The kinetic parameters for the incorporation of both compound I and the natural substrate dATP were determined on the basis of the single completed hit model [21, 22]. P₁ and T₁ were used as the primer and template duplex, respectively, HIV-1 RT was used as enzyme. The kinetic parameters K_M and V_max and the derived value V_max/K_M are given in Table 4.

Table 4 The kinetic parameters for incorporation of the natural nucleotide (dAMP) and compound I into P₁T₁ by HIV-1 RT.

As seen in Table 4, compound I has the similar V_max value as the natural substrate dATP. However, a large increase in the K_M value for the incorporation of compound I was observed, this implies a decrease in the enzymatic affinity for dinucleoside pyrophosphate compared to dATP. Thus, the measured ratio (V_max/K_M) toward HIV-1 RT is 1400-fold decreased compared to that of dATP. It can be assumed that although compound I was rather efficient as a substrate into a growing DNA strand, the enzyme still prefer the natural dATP as substrate when giving a choice.

Stability studies

In an earlier enzymatic incorporation study when thermostable DNA polymerases were used, it was observed that the chemical stability of dNTP analogues at elevated temperature (70°C) influenced the efficiency of incorporation [9]. In this study we have determined the chemical stability of dAppdA, dGppdG, dCppdC, dTppdT and dGppdC analogues in the pH range 2-13 at room temperature (25°C) and 70°C using NMR spectroscopy. All the analogues are found to be stable (no degradation product identified after 3 days) in pH range 7-12 at 25°C and 70°C. Generally dNppdN analogues are very stable, and they degrade to nucleotides and nucleosides slowly above pH 12 at 70°C and degrade rapidly at less than pH 3, 70°C. In comparison the N-glycosidic bond of dAppdA, dGppdG, dCppdC analogues is weaker than the P-O-P linkage in acidic medium, since degradation of glycosidic bond was observed in pH range 4-6.5 at 70°C.

Relevance to prebiotic chemistry

For protein synthesis, Nature uses the principle of putting information (nucleotide triplets) in the leaving group (tRNA). This is necessary because during protein synthesis, information needs to be translated from one biopolymer (RNA) to another (proteins). Putting information in the leaving group may be a general approach to copy information and to diversify information in case the polymer that is synthesized (oligonucleotide) is the same as the polymer that is used as information system (oligonucleotide): e.g. a tRNA that is loaded with a nucleotide triplet instead of amino acids, could have been purposely designed by Nature for this goal. Such a system may represent an example of autocatalytic self-replication avoiding product inhibition [23] (when the dinucleotide is built from complementary bases, Scheme 2a, Figure 7) and of the generation of new information (when the dinucleotide is built from non-complementary bases, Scheme 3, Figure 8) and would generate molecular networks (Figures 7 and 8). Larger polymers may be formed from shorter oligomers by a ligase reaction (Scheme 4).

Scheme 2

Reaction products that may be formed using AppU as reagent and pApA as template.

Figure 7

Hypothetical metabolic cycles of compounds (UppApA and AppUpU) that may catalyze their own formation (from UppA) and may catalyze the formation of their complement. The reaction products that are not shown (pA and pU) may be reused after condensation.

Scheme 3

Reaction products that may be formed using UppC and UppG as reagent and pApA as template.

Figure 8

Hypothetical metabolic cycles of four dimers (ZppXpY) as generated from two non-selfcomplementary building blocks (CppA and UppG). The reaction products that are not shown (pU, pG, pA, pC) may be reused after condensation.

Scheme 4

Ligase reaction may lead to larger information systems.

The process may be run without waste of material as the nucleotide leaving group is reusable (condensation of pX and pY to the XppY reagent in the presence of an activating agent).

It is obvious that for every reaction there will be a competition between phosphodiester formation between the nucleotides away from the template (Scheme 2a) and between the nucleotides recognized by the template (Scheme 2b).

In the latter case (Scheme 2b, Scheme 3b), we end up in the classical trap of product inhibition. In the first case (Scheme 2a), exponential growth of information without product inhibition may become possible.

Using 4 nucleotides, however, in total 10 possible pyrophosphate linked dinucleotides can be formed from which 4 can be recognized by a homodimer, which makes it unlikely that a process including all 4 nucleotides would have an advantage for prebiotic synthesis of nucleic acids (when compared with 4 nucleoside triphosphates).

Once a diverse set of short oligomers is formed in this way, they can be assembled via Watson-Crick base pairing and grow to a larger self-complementary information system (Scheme 4).

However, given the high stability of the pyrophosphate linker, it will not be easy to find an appropriate catalyst for these reactions.

For an experimental set-up, the selection of the interphosphate linking atom X (Figure 9, X which could be O, NH, S or even imidazole or a larger molecule) and the selection of the reaction circumstances (pH, salt, temperature) to perform dimerization is not easy to make. The properties of X are important. When both nucleotides are bound to X in a different way (for example, via an ester and amide linkage), the direction of the dimerization process can be controlled.

Figure 9

Structure of a pyrophosphate linked nucleotide dimer.

Conclusions

The experimental set-up in this monograph has demonstrated that initial DNA dependent DNA polymerase activity can be ascribed to HIV-RT and several thermostable DNA polymerases using dinucleoside pyrophosphates as substrates. This means that, when using such heterodimers for DNA synthesis (for example dAppdT and dGppdC), theoretically, it is possible to synthesize DNA enzymatically using two building blocks instead of four. These initial experiments have been carried out in the light of trying to find model compounds that could have been used in a primitive life form to build up an information system while still having the capacity for exponential growth of this information.

The general principle that has been used by Nature for protein synthesis, i.e., to incorporate information in leaving groups, may as well have been used by Nature to select its most abundant information system itself. Future experiments are aimed to find the appropriate X (Figure 9) together with the optimal reaction circumstances to realize metabolic cycles as depicted in Figures 7 and 8.

Experimental Section

General

For all reactions, chemicals of analytical or synthetic grade were obtained from commercial sources and were used without purification. Technical solvents were obtained from Brenntag (Deerlijk, Belgium). Analytical thin Layer Chromatography was performed on Alugram^® silica gel UV254 mesh 60, 0.20 mm (Macherey-Nagel). NMR Spectra were recorded on a Brucker Avance™ II 300 MHz or 500 MHz NMR spectrometer. Chemical shifts are expressed as δ units (part per million) down field from TMS (tetramethyl silane) for ¹H and ¹³C. ³¹P NMR chemical shifts are referenced to an external 85% H₃PO₄ standard (δ = 0.00 ppm). Exact mass spectra were obtained with a quadrupol/orthogonal-acceleration time-of-flight tandem mass spectrometer (Q-Tof 2, Micromass) equipped with a standard electrospray ionization (ESI) source. HPLC was performed on Waters 1525-2487 system using C18 column by a gradient elution of acetonitrile and 50 mM triethylammonium bicarbonate (TEAB) buffer.

Synthesis of deoxyadenosine-5'-phosphoroimidazole

General Procedure (I): Deoxyadeosine-5'-monophosphate (100 mg, 0.30 mmoles) was dissolved in dry DMSO (2 mL), pulverized triphenylphosphine (327 mg, 0.96 mmoles), 2,2-dipyridyl disulfide (211 mg, 0.96 mmoles) and imidazole (327 mg, 4.8 mmoles) were added to the solution, the resulting yellow solution was stirred at room temperature for 50 min, after completion the mixture was poured into 0.1 M sodium iodide solution in cold acetone (30 mL). The white precipitate was collected by centrifugation and washed several times with cold fresh acetone. The product was dried in desicator for 1 h.; the white solid was stored at -20°C (99 mg, yield 87%). ¹H NMR (500 MHz, D₂O): δ= 8.07 (s, 1H, H₈), 7.92 (s, 1H, H₂), 6.20 (t, 1H, J = 6.2 Hz, H_1'), 4.55 (m, 1H, H_3'), 4.13 (m, 3H, H_4'+H_5'), 2.50 (m, 1H, H_2'a), 2.43 (m, 1H, H_2'b); ¹³C NMR (125 MHz, D₂O): δ = 155.1, 152.6, 148.4, 140.1, 118.4, 86.5, 84.4, 71.2, 66.0, 40.0; ³¹P NMR (121 MHz, D₂O): δ = -11.23; HRMS: [M-H]^- calculated for C₂₀H₂₅N₁₀O₁₁P₂ 643.1185, found: 643.1183.

Synthesis of P¹, P²-bis(2'-deoxyadenosin-5'-yl) Pyrophosphate (dAppdA)

General Procedure (II): A solution of 2'-deoxyadeonosine-5'-monophosphate (100 mg, 0.30 mmoles), 2'-deoxyadeonosine-5'-phosphorimidazolide (114 mg, 0.30 mmoles), zinc chloride (35 mg, 0.30 mmoles) in 0.2 M N-ethylmorpholine buffer (3 mL, pH 7.5) was stirred at room temperature under argon for 2 days. The reaction was monitored by TLC (i-PrOH/NH₃/H₂O 7:1:2) and ³¹P NMR, quenched the reaction with 0.25 M ethylenediaminetetraacetic acid (EDTA) in order to breakdown the nucleotide-metal complex, then the mixture was lyophilized affording a white solid. The product was isolated by HPLC purification on a C18 column, running with a gradient of CH₃CN in 50 mM triethylammonium (TEAB) buffer. Yield 28% (from HPLC purification profile). ¹H NMR (500 MHz, D₂O): δ = 8.02 (s, 2H, H₈), 7.90 (s, 2H, H₂), 6.16 (t, 2H, J = 5.7 Hz, H_1'), 4.49 (m, 2H, H_3'), 4.07 (m, 4H, H_5'), 4.02 (m, 2H, H_4'), 2.46 (m, 2H, H_2'a), 2.40 (m, 2H, H_2'b); ¹³C NMR (125 MHz, D₂O): δ = 155.1, 152.3, 148.4, 140.1, 118.4, 86.1, 84.1, 71.2, 66.0, 40.0; ³¹P NMR (121 MHz, D₂O): δ = -11.27; HRMS: [M-H]^- calculated for C₂₀H₂₅N₁₀O₁₁P₂ 643.1185, found: 643.1183.

Synthesis of P¹, P²-bis(2'-deoxythymidin-5'-yl) Pyrophosphate (dTppdT)

The general procedure (I) was applied using thymidine-5'-monophosphate (300 mg, 0.93 mmoles), triphenylphosphine (1.01 g, 2.98 mmoles), 2,2-dipyridyl disulfide (655 mg, 2,98 mmoles) and imidazole (1.02 g, 14.9 mmoles). After reaction the obtained product was applied to general procedure (II) using thymidine-5'-monophosphate (100 mg, 0.31 mmoles), thymidine-5'-phosphorimidazolide (115 mg, 0.31 mmoles) and zinc chloride (36 mg, 0.31 mmoles). After purification obtained white solid product (yield 35% (from HPLC purification profile)). ¹H NMR (500 MHz, D₂O): δ = 7.71 (s, 2H, H₆), 6.29 (t, 2H, J = 7.0 Hz, H_1'), 4.56-4.61 (m, 2H, H_3'), 4.16-4.17 (m, 2H, H_4'), 4.12-4.13 (m, 4H, H_5'), 2.32-2.36 (m, 4H, H_2'), 1.91 (s, 6H, CH₃); ¹³C NMR (125 MHz, D₂O): δ = 166.1, 151.3, 137.1, 111.4, 85.0, 84.9, 70.5, 65.0, 38.3, 11.4; ³¹P NMR (121 MHz, D₂O): δ = -11.62; HRMS: [M-H]^- calculated for C₂₀H₂₈N₄O₁₅P₂ 625.0953, found: 625.0962

Synthesis of P¹-(2'-deoxyadenosin-5'-yl) P²-(2'-deoxythymidin-5'-yl) Pyrophosphate (dAppdT)

The general procedure (II) was applied using 2'-deoxyadeonosine- 5'-phosphorimidazolide (100 mg, 0.26 mmoles) and thymidine-5'-monophosphate (85 mg, 0.26 mmoles) and zinc chloride (30 mg, 0.26 mmoles). After purification obtained white solid product (yield 18% (from HPLC purification profile)) ¹H NMR (500 MHz, D₂O): δ = 8.36 (s, 1H, H₈-A), 8.10 (s, 1H, H₂-A), 7.31 (s, 1H, H₆-T), 6.39 (t, 1H, J = 6.0 Hz, H_1'-A), 6.15 (t, 1H, J = 6.0 Hz, H_1'-T), 4.63-4.65 (m, 1H, H_3'-A), 4.40-4.42 (m, 1H, H_3'-T), 4.16-4.18 (m, 1H, H_4'-A), 4.07-4.00 (m, 5H, H_4'-T, H_5'-A, H_5'-T), 2.71-2.75 (m, 1H, H_2'a-A), 2.46-2.50 (m, 1H, H_2'a-T), 2.11-2.15 (m, 2H, H_2'b-T), 2.04-2.08 (m, 2H, H_2'b-A), 1.67 (s, 3H, T-CH₃); ¹³C NMR (125 MHz, D₂O): δ = 181.4, 172.3, 155.3, 152.4, 148.5, 139.7, 136.1, 118.4, 111.4, 85.6, 84.8, 84.6, 83.3, 71.0, 71.1, 65.4, 65.3, 38.9, 38.6, 12.3; ³¹P NMR (121 MHz, D₂O): δ = -11.41; HRMS: [M-H]^- calculated for C₂₀H₂₆N₇O₁₃P₂ 634.1069, found: 634.1063.

Synthesis of P¹, P²-bis(2'-deoxyguanosin-5'-yl) Pyrophosphate (dGppdG)

The general procedure (I) was applied using using 2'-deoxyguanosine-5'-monophosphate (300 mg, 0.78 mmoles), triphenylphosphine (850 mg, 2.50 mmoles), 2,2-dipyridyl disulfide (549 mg, 2,50 mmoles) and imidazole (850 mg, 12.9 mmoles). After reaction the obtained product was applied to general procedure (II) using 2'-deoxyguanosine-5'-monophosphate (100 mg, 0.26 mmoles), 2'-deoxyguanosine-5'-phosphorimidazolide (102 mg, 0.26 mmoles) and Pd(NO₃)₂ (60 mg, 0.36 mmoles). After purification obtained white solid product (yield 33% (from HPLC purification profile)). ¹H NMR (500 MHz, D₂O): δ = 7.86 (s, 2H, H₆), 6.11 (t, 2H, J = 6.5 Hz, H_1'), 4.54-4.56 (m, 2H, H_3'), 4.05-4.07 (m, 2H, H_4'), 4.03-4.05 (m, 4H, H_5'), 2.54-2.63 (m, 2H, H_2'a), 2.33-2.36 (m, 2H, H_2'b); ¹³C NMR (125 MHz, D₂O): δ = 162.1, 156.3, 150.8, 136.3, 116.3, 85.1, 83.0, 70.8, 65.2, 38.4; ³¹P NMR (121 MHz, D₂O): δ = -11.40; HRMS: [M-H]^- calculated for C₂₀H₂₅N₁₀O₁₃P₂ 675.1083, found: 675.1092.

Synthesis of P¹-(2'-deoxyguanosin-5'-yl) P²-(2'-deoxycytidin-5'-yl) Pyrophosphate (dGppdC)

The general procedure (I) was applied using using 2'-deoxyguanosine-5'-monophosphate (300 mg, 0.78 mmoles), triphenylphosphine (850 mg, 2.50 mmoles), 2,2-dipyridyl disulfide (549 mg, 2,50 mmoles) and imidazole (850 mg, 12.9 mmoles). After reaction the obtained product was applied to general procedure (II) using 2'-deoxyguanosine-5'-phosphorimidazolide (100 mg, 0.25 mmoles), 2'-deoxycytidine-5'-monophosphate (82 mg, 0.25 mmoles) and Pd(NO₃)₂ (58 mg, 0.25 mmoles). After purification obtained white solid product (yield 15% (from HPLC purification profile)). ¹H NMR (500 MHz, D₂O): δ = 8.04 (s, 1H, H₈-G), 7.82 (d, 1H, J = 7.5 Hz, H₆-C), 6.30 (t, 1H, J = 7.0 Hz, H_1'-G), 6.25 (t, 1H, J = 6.5 Hz, H_1'-C), 5.97 (d, 1H, J = 7.0 Hz, H₅-C), 4.63-4.67 (m, 1H, H_3'-G), 4.52-4.54 (m, 1H, H_3'-C), 4.23-4.25 (m, 1H, H_4'-G), 4.15-4.17 (m, 1H, H_4'-C), 4.12-4.15 (m, 4H, H_5'-G, H_5'-C), 2.77-2.82 (m, 1H, H_2'a-G), 2.48-2.50 (m, 1H, H_2'a-C), 2.36-2.41 (m, 1H, H_2'b-C), 2.18-2.24 (m, 1H, H_2'b-G); ¹³C NMR (125 MHz, D₂O): δ = 165.6, 161.5, 156.9, 150.9, 140.9, 136.5, 116.3, 95.9, 85.5, 85.2, 85.0, 83.0, 70.9, 70.2, 65.3, 64.8, 39.2, 38.1; ³¹P NMR (121 MHz, D₂O): δ = -11.40; HRMS: [M-H]^- calculated for C₁₉H₂₅N₈O₁₃P₂ 635.1022, found: 635.1099.

Synthesis of P¹-(2'-deoxyadenosin-5'-yl) P²-(2'-deoxythymidin-5'-yl) Pyrophosphate (dAppdC)

The general procedure (II) was applied using 2'-deoxyadeonosine-5'-phosphorimidazolide (100 mg, 0.26 mmoles), 2'-deoxycytidine-5'-monophosphate (85 mg, 0.26 mmoles) and zinc chloride (30 mg, 0.26 mmoles). After purification obtained white solid product (yield 18% (from HPLC purification profile)). ¹H NMR (500 MHz, D₂O): δ = 8.38 (s, 1H, H₈-A), 8.10 (s, 1H, H₂-A), 7.53 (d, 1H, J = 6.0 Hz, H₆-C), 6.40 (t, 1H, J = 5.5 Hz, H_1'-A), 6.08 (t, 1H, J = 5.5 Hz, H_1'-C), 5.68 (d, 1H, J = 6.5 Hz, H₅-C), 4.65-4.67 (m, 1H, H_3'-A), 4.37-4.39 (m, 1H, H_3'-C), 4.20-4.21 (m, 1H, H_4'-A), 4.09-4.05 (m, 5H, H_5'-A, H_5'-C, H_4'-C), 2.73-2.78 (m, 1H, H_2'a-A), 2.47-2.51 (m, 1H, H_2'a-C), 2.22-2.26 (m, 1H, H_2'b-C), 1.99-2.03 (m, 1H, H_2'b-A); ¹³C NMR (125 MHz, D₂O): δ = 165.5, 157.0, 155.3, 152.4, 148.5, 140.6, 139.6, 118.3, 95.8, 85.7, 85.6, 85.2, 83.4, 71.2, 70.4, 65.5, 65.1, 39.5, 38.8; ³¹P NMR (121 MHz, D₂O): δ = -11.34; HRMS: [M-H]^- calculated for C₂₀H₂₅N₈O₁₂P₂ 619.1073, found: 619.1089

Synthesis of P¹, P²-bis(2'-deoxycytidin-5'-yl) Pyrophosphate (dCppdC)

The general procedure (I) was applied using 2'-deoxycytidine-5'-monophosphate (300 mg, 0.93 mmoles), triphenylphosphine (1.01 g, 2.98 mmoles), 2,2-dipyridyl disulfide (655 mg, 2,98 mmoles) and imidazole (1.02 g, 14.9 mmoles). After reaction the obtained product was applied to general procedure (II) using 2'-deoxycytidine-5'-monophosphate (100 mg, 0.31 mmoles), 2'-deoxycytidine-5'-phosphorimidazolide (115 mg, 0.31 mmoles) and zinc chloride (36 mg, 0.31 mmoles). After purification obtained white solid product (yield 26% (from HPLC purification profile)). ¹H NMR (500 MHz, D₂O): δ = 7.90 (d, J = 7.6 Hz, 2H, H₆), 6.31 (t, 2H, J = 6.8 Hz, H_1'), 6.05 (d, 2H, J = 7.5 Hz, H₅), 4.52-4.56 (m, 2H, H_3'), 4.14-4.18 (m, 6H, H_4'+H_5'), 2.35-2.41 (m, 2H, H_2'a), 2.19-2.28 (m, 2H, H_2'b); ¹³C NMR (125 MHz, D₂O): δ = 165.7, 157.2, 141.2, 96.2, 85.5, 85.0, 70.4, 64.9, 39.2; ³¹P NMR (121 MHz, D₂O): δ = -8.84; HRMS: [M-H]^- calculated for C₁₈H₂₅N₆O₁₃P₂ 595.0960, found: 595.0954.

Oligodeoxyribonucleotides preparation

Oligodeoxyribonucleotides P₁, P₂, T₁-T₆ were purchased from Sigma Genosys. The concentrations were measured with a Varian Cary-300-Bio UV spectrophotometer. The lyophilized oligonucleotides were dissolved in diethylpyrocarbonate (DEPC)-treated water and stored at -20°C. The primer oligonucleotides were 5'-³³P-labeled with 5'-[γ³³P]-ATP (Perkin Elmer) using T₄ polynucleotide kinase (NEB) according to standard procedures. The labelled oligonucleotide was further purified using Illustra TM Microspin TM G-25 columns (GE Healthcare).

DNA polymerase reactions

End-labelled primer was annealed to its template by combining primer and template in a molar ratio of 1:2 and heating the mixture to 70°C for 10 min followed by slow cooling to room temperature over a period of 1.5 h. For the incorporation of compound I-VII, a series of 20 μL-batch reactions were performed with the enzyme (HIV-1 RT, Taq, Vent (exo^-), Therminator DNA polymerase). The final mixture contained 125 nM primer template complex, RT buffer (250 mM Tris.HCl, 250 mM KCl, 50 mM MgCl₂, 2.5 mM spermidine, 50 mM dithiothreitol (DTT); pH 8.3), appropriate concentration of enzyme, and different concentrations of dinucleoside diphosphates building blocks (1 mM, 500 μM, 200 μM and 100 μM). In the control reaction a 10 μM dATP was used as reference. The mixture was incubated at 37°C or 75°C respectively, and aliquots were quenched after 10, 20, 30, 60 and 120 min. For elongation experiments, the same mixture with appropriate primer/template hybrid was incubated at 37°C or 75°C and aliquots were quenched after 15, 30, 60, 90, 120 min.

Steady-state kinetics of single nucleotide incorporation

The steady-state kinetics of single nucleotide incorporation of compound I and the natural nucleoside triphosphate (dATP) was determined by a gel-based polymerase assay. In all the experiments, the template T₁ and the primer P₁ were used. The primer and template in a 1:2 molar ratio were hybridised in a buffer containing 20 mM Tris.HCl, 10 mM KCl, 2 mM MgSO₄, 0.1% Triton X-100, pH 8.3 and used in an amount to provide 125 nM concentration of the primer in each 20 μL reaction. A range of building block concentrations between 10 μM and 1 mM for the phosphoramidate and between 0.1 μM and 10 μM for the natural building block was used. The final concentrations of primer-template complex and HIV-1 RT were 125 nM, and 0.025 U/μL, respectively. Reaction mixtures were incubated at 37°C and aliquots were drawn at 6 different time intervals. The reactions were quenched by addition of a buffer containing 80% formamide, 2 mM EDTA and 1X TBE buffer. The analysis of polymerase reaction was performed by polyacrylamide gel electrophoresis (see detailed protocol below). The incorporation rates (V) were calculated based on the percentage of the extended oligonucleotide in the mixture (P+1 band). The kinetic parameters (V_Max and K_M) were determined by plotting V (nM/min^-1) versus substrate concentration (μM) and fitting the data to a non-linear Michaelis-Menten regression using GraphPad Prism Software version 5.0.

Electrophoresis

All polymerase reaction aliquots (2.5 μL) were quenched by the addition of 10 μL of loading buffer (90% formamide, 0.05% bromophenol blue, 0.05% xylene cyanol and 50 mM ethylenediaminetetraacetic acid). Samples were heated at 85°C for 3 minutes prior to analysis by electrophoresis for 2.5 h at 2000 V on a 30 cm × 40 cm × 0.4 mm 20% (19:1 mono:bis) denaturing gel in the presence of a 100 mM Tris-borate, 2.5 mM EDTA buffer; pH 8.3. Products were visualised by phosphorimaging. The amount of radioactivity in the bands corresponding to the products of enzymatic reactions was determined by using the imaging device Cyclone^® and the Optiquant image analysis software (PerkinElmer).