Table 3 Single incorporation results of compound III-VII by Taq, Vent (exo-) and Therminator DNA polymerase.
From: Enzymatic synthesis of DNA employing pyrophosphate-linked dinucleotide substrates
Compound No. | Structure | Primer/Template duplex | Single incorporation result, % | ||
|---|---|---|---|---|---|
Taq | Vent (exo - ) | Therminator | |||
III | dAppdT | P1/T1 | 0% | 0% | 59% |
III | dAppdT | P1/T2 | 0% | 0% | 55% |
IV | dGppdG | P1/T4 | 0% | 0% | 74% |
V | dGppdC | P1/T4 | 0% | 0% | 48% |
V | dGppdC | P2/T5 | 0% | 0% | 0% |
VI | dAppdC | P1/T1 | 0% | 0% | 81% |
VI | dAppdC | P2/T5 | 0% | 0% | 0% |
VII | dCppdC | P2/T5 | 0% | 0% | 0% |