Functional effect of the removed reactions that decrease the RAF-size by more than 100. There were 13 reactions involving the synthesis of composite cofactors that reduced the RAF size by more than 100 reactions that are not shown in the Figure. These reactions often comprised the coupling of, for example, folate and magnesium, or thiamine and magnesium, i.e., reactions in which more than one cofactor was required. They were removed from the list so that only E. coli reactions and not those generated by recoding of the data are represented. An additional seven reactions in the list that resulted solely from the use of different designations for the same compound in the E. coli metabolic network and the E. coli Uniprot database were also excluded.