Skip to main content

Table 4 The kinetic parameters for incorporation of the natural nucleotide (dAMP) and compound I into P1T1 by HIV-1 RT.

From: Enzymatic synthesis of DNA employing pyrophosphate-linked dinucleotide substrates

  Vmax
[nM.min-1]
KM
[μM]
Vmax/KM
[min-1]
dATP 35 ± 2 0.3 ± 0.1 116 × 10-3
Compound I 37 ± 5 447 ± 117 0.08 × 10-3
  1. [HIV-1 RT] = 0.025 U/μL, [P1T1] = 125 nM.