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Journal of Systems Chemistry

Table 4 The kinetic parameters for incorporation of the natural nucleotide (dAMP) and compound I into P1T1 by HIV-1 RT.

From: Enzymatic synthesis of DNA employing pyrophosphate-linked dinucleotide substrates

 

Vmax

[nM.min-1]

KM

[μM]

Vmax/KM

[min-1]

dATP

35 ± 2

0.3 ± 0.1

116 × 10-3

Compound I

37 ± 5

447 ± 117

0.08 × 10-3

  1. [HIV-1 RT] = 0.025 U/μL, [P1T1] = 125 nM.
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